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Production of red fluorescent protein (mCherry) in an inducible E. coli expression system in a bioreactor, purification and characterization

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dc.contributor.author Kuduğ, Hülya
dc.contributor.author Ataman, Bahadır
dc.contributor.author İmamoğlu, Rizvan
dc.contributor.author Düzgün, Duygu
dc.contributor.author Gökçe, İsa
dc.date.accessioned 2019-07-16T08:17:10Z
dc.date.available 2019-07-16T08:17:10Z
dc.date.issued 2018-06-01
dc.identifier.uri https://dergipark.org.tr/iarej/issue/44303/429547
dc.identifier.uri http://hdl.handle.net/11772/1656
dc.description.abstract New and improved genetic engineered variants of fluorescent proteins (FPs) have become useful tools for bioimaging in biomedical researches. Red fluorescent proteins (RFPs) first derived from the sea anemone Discosoma show high performance in vivo labeling and imaging. mCherry is a member of RFPs which has very high photostability, resistant to photo bleaching and rapid maturation. These advantages ensure that mCherry can be successfully fused to many proteins and widely used for quantitative imaging techniques. In this study, the constructed recombinant plasmid pBADCherry was expressed in Escherichia coli BL21(AI) then culture conditions, inducer concentration and induction time were optimized. Results of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis demonstrated that 5 hours induction at 0.04% of arabinose concentration was optimal for the highest mCherry yield. The expression of hexa histidine-tagged (6xHis) recombinant mCherry was induced by arabinose and purification performed using nickel (Ni2+) affinity chromatography. High throughput expression of 81 mg fluorescent protein from a liter of E. coli culture carried out in bioreactor. en_US
dc.language.iso eng en_US
dc.publisher International Advanced Researches And Engineering Journal en_US
dc.rights info:eu-repo/semantics/openAccess en_US
dc.subject Bioreactor en_US
dc.subject E. coli en_US
dc.subject Fluorescent proteins en_US
dc.subject mCherry en_US
dc.subject pBAD en_US
dc.title Production of red fluorescent protein (mCherry) in an inducible E. coli expression system in a bioreactor, purification and characterization en_US
dc.type article en_US
dc.relation.journal International Advanced Researches And Engineering Journal en_US
dc.contributor.department Barın Üniversitesi, Fen Fakültesi, Biyoteknoloji Bölümü en_US
dc.identifier.volume 03 en_US
dc.identifier.issue 01 en_US
dc.identifier.startpage 020 en_US
dc.identifier.endpage 025 en_US


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