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dc.contributor.authorKocyigit, Umit M.
dc.contributor.authorTaslimi, Parham
dc.contributor.authorGulçin, İlhami
dc.date.accessioned2019-04-29T07:51:40Z
dc.date.available2019-04-29T07:51:40Z
dc.date.issued2018-08
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/full/10.1002/jbt.22172
dc.identifier.urihttp://hdl.handle.net/11772/1122
dc.description.abstractIn this work, the carbonic anhydrase (CA) enzyme was purified from Kangal Akkaraman sheep in Sivas, Turkey with specific activity value of 6681.57 EU/mg and yield of 14.90% with using affinity column chromatography. For designating the subunit molecular mass and enzyme purity. sodium dodecyl sulfate polyacrylamide gel electrophoresis method was used and single band for this procedure was obtained. The molecular mass of CA enzyme was found as 28.89 kDa. In this study, the optimum temperature and optimum pH were obtained from 30 and 7.5. V-max and K(m )values for p-nitrophenylacetate substrate of the CA were determined from Lineweaver-Burk graphs. Additionally, the inhibitory results of diverse heavy metal ions (He+, Fe2+, pb(2+), co(2+), A(g+), and Cu2+) on sheep were studied. Indeed, CA enzyme activities of Kangal sheep were investigated with using esterase procedure under in vitro conditions. The heavy metal concentrations inhibiting 50% of enzyme activity (IC50) and K-i values were obtained.en_US
dc.language.isoengen_US
dc.publisherWileyen_US
dc.relation.isversionof10.1002/jbt.22172en_US
dc.rightsinfo:eu-repo/semantics/restrictedAccessen_US
dc.subjectKangal Akkaraman sheepen_US
dc.subjectCarbonic anhydraseen_US
dc.subjectCharacterizationen_US
dc.subjectEnzyme purificationen_US
dc.titleCharacterization and inhibition effects of some metal ions on carbonic anhydrase enzyme from Kangal Akkaraman sheepen_US
dc.typearticleen_US
dc.relation.journalJournal of Biochemical and Molecular Toxicologyen_US
dc.contributor.departmentBartın Üniversitesi, Fen Fakültesi, Biyoteknoloji Bölümüen_US
dc.identifier.volume32en_US
dc.identifier.issue8en_US
dc.identifier.startpagee22172en_US


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