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dc.contributor.authorTaslimi, Parham
dc.contributor.authorAslan, Hatice Esra
dc.contributor.authorDemir, Yeliz
dc.contributor.authorOztaskin, Necla
dc.contributor.authorMaraş, Ahmet
dc.contributor.authorGulçin, İlhami
dc.contributor.authorBeydemir, Sukru
dc.contributor.authorGoksu, Suleyman
dc.date.accessioned2019-05-06T13:27:33Z
dc.date.available2019-05-06T13:27:33Z
dc.date.issued2018-11
dc.identifier.urihttp://hdl.handle.net/11772/1155
dc.description.abstractDiabetes mellitus (DM) is a chronic metabolic disease in which there are high blood sugar levels over a prolonged period. Aldose reductase (AR) belongs to aldo-keto reductase superfamily and plays a key role in the polyol pathway. alpha-Glycosidase and alpha-amylase are important enzymes in glucose metabolism. In this study, AR was purified from purified from cow liver. The enzyme was obtained with 139.17 purification fold and with a specific activity of 1.67 EU/mg protein. Then, it is observed the inhibition effect of diarylmethanons (1a-d), bromophenols (2a-d and 4a-d) and diarylmethanes (3a-d) on aldose reductase, a-glycosidase and alpha-amylase enzymes. In these series, compound 2a showed lowest inhibitory activity against AR with a K-i value of 1.09 +/- 0.29 mu M while compound 2d showed highest inhibitory activity against AR with a K-i value of 0.092 +/- 0.015 mu M. Additionally, alpha-glycosidase and alpha-amylase enzymes were easily inhibited by these compounds. All compounds were tested for their inhibition effects against of alpha-glycosidase enzyme and demonstrated efficient inhibition profiles with K-i values in the range of 14.44 +/- 0.88-43.53 +/- 9.06 nM, and IC50 values in the range of 11.72-46.11 nM. Also, inhibition of the alpha-amylase enzyme, which determined an effective inhibition profile with IC50 values, is in the range of 3.84-29.61 nM. (C) 2018 Elsevier B.V. All rights reserved.en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.isversionof10.1016/j.ijbiomac.2018.08.004en_US
dc.rightsinfo:eu-repo/semantics/restrictedAccessen_US
dc.subjectAldose reductaseen_US
dc.subjectBromophenolsen_US
dc.subjectDiarylmethanonsen_US
dc.subjectAlpha-amylaseen_US
dc.subjectAlpha-glycosidaseen_US
dc.titleDiarylmethanon, bromophenol and diarylmethane compounds: Discovery of potent aldose reductase, α-amylase and α-glycosidase inhibitors as new therapeutic approach in diabetes and functional hyperglycemiaen_US
dc.typearticleen_US
dc.relation.journalInternational Journal of Biological Macromoleculesen_US
dc.contributor.departmentBartın Üniversitesi, Fen Fakültesi, Biyoteknoloji Bölümüen_US
dc.identifier.volume119en_US
dc.identifier.startpage857en_US
dc.identifier.endpage863en_US


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