Alleviation of copper stress with homobrassinosteroid in germinating sunflower roots
Özet
We investigated potential alleviation effects of
homobrassinosteroid (HBR) hormone on sunflower
(Helianthus annuus L. cv. TR003) grown under
copper (Cu) stress conditions. Seeds were grown
under Cu (30 µM and 40 µM) and also HBR (2
µM) treatments to analyse primary root lengths,
protein contents, antioxidant system enzymes'
activities (superoxide dismutase-SOD and catalaseCAT) and expressions of these enzymes at 48 h and
72 h. When compared to control, Cu applications
decreased primary root lengths. On the other hand,
HBR application increased primary root lengths of
Cu-stressed samples at 48 h and 72 h. Depending
on concentration and timing, protein contents and
enzyme activities showed varying results in Cutreated and Cu+HBR-applied samples. In addition
to enzyme activities, SOD and CAT expression
profiles were also investigated by RT-PCR.
Expected band sizes for SOD (309 bp) and CAT
(248 bp) were observed in all samples. These
results indicate a positive role of HBR on Custressed sunflower for agricultural applications.
