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dc.contributor.authorÇelebioğlu, Hasan Ufuk
dc.contributor.authorKarakurt, Serdar
dc.contributor.authorGençler-Özkan, Ayşe Mine
dc.contributor.authorŞen, Alaattin
dc.contributor.authorAdalı, Orhan
dc.date.accessioned2019-05-17T13:49:26Z
dc.date.available2019-05-17T13:49:26Z
dc.date.issued2012-06-29
dc.identifier.citationCelebioglu, H. U., Karakurt, S., Gencler-Ozkan, A. M., Sen, A., Adali, O. 2012. In vivo effects of o-coumaric acid on rat liver flavin-containing monooxygenase (FMO) activity and expression. Poster presentation at 10th International Symposium on Pharmaceutical Sciences, Ankara-Türkiye, 26-29 Haziran 2012. Book of Abstracts, pp 342en_US
dc.identifier.urihttp://hdl.handle.net/11772/1227
dc.description.abstractPhenolic compounds are biologically active molecules and suggested to have some disease-preventive properties such as cardiovascular and certain types of cancer. Coumaric acid has been known as a phenolic compound and found in a wide variety of plants such as peanuts, tomatoes, carrots, and garlic and form the part of human diet; function as antioxidant and chemopreventive agents. 1-2 Flavin-containing monooxygenases (FMOs, EC 1.14.13.8) are phase I enzymes, responsible for oxygenation of a wide range of nucleophilic heteroatom-containing xenobiotics such as drugs, pesticides, neurotoxins, and other chemicals. They contain FAD in their structure and use NADPH as cofactor and molecular oxygen for their reactions. FMOs can be affected by hormonal and dietary components.3 The aim of this study was to determine the in vivo effects of o-coumaric acid on rat liver FMO activity, protein and mRNA expressions. 30 mg/kg of body weight of o-coumaric acid was intraperitonally injected to 14 albino Wistar rats, while 8 rats were used as control. After 9 days of injection, the rats were decapitated and microsomal fractions of livers were prepared using homogenization and differential centrifugation. FMO enzyme activity of microsomal samples was assayed by using methimazole as substrate. Protein and mRNA expressions were carried out by Western Blot and quantitative Real-Time PCR techniques respectively. The results showed that FMO activity and protein expression were increased 48% and 34%, respectively, by o-coumaric acid injection compared to controls (p<0.05). In addition, o-coumaric acid injection increased the mRNA expression of FMO as 2.1-fold compared to controls (p<0.05). In conclusion, our data suggest that o-coumaric acid found in diet may induce FMO expression and FMO activity enhanced metabolism of drugs, pesticides, and other xenobiotics.en_US
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectO-Coumaric aciden_US
dc.subjectFlavin-containing monooxygenaseen_US
dc.subjectFMOen_US
dc.subjectmRNA and protein expressionen_US
dc.subjectRat liveren_US
dc.titleIn vivo effects of o-coumaric acid on rat liver flavin-containing monooxygenase (fmo) activity and expressionen_US
dc.typeconferenceObjecten_US
dc.relation.journal10th International Symposium on Pharmaceutical Sciencesen_US
dc.contributor.departmentBartın Üniversitesi, Fen Fakültesi, Biyoteknoloji Bölümüen_US
dc.identifier.startpage342en_US


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