Anti-oxidant, anti-elastase, anti-collagenase, anti-hyaluronidase, anti-breast cancer activities of some natural compounds

Abstract

Natural products provide great potential for contemporary medication development due to their diverse biological activity, large range of sources, and structural diversity. In this investigation, the enzymes collagenase, elastasei and hyaluronidase were inhibited by Clusiacyclol A, Cycloolivil, and Cyclopenol molecules with excellent to good IC50 values of 11.35 ± 1.24, 75.36 ± 11.73, and 25.17 ± 3.38 µM for Clusiacyclol A, and 19.42 ± 2.66, 84.16±6.80, and 94.36± 8.46 µM for Cycloolivil, and 0.65±0.04, 50.24±5.07, and 66.01± 10.83 µM for Cyclopenol, respectively. These chemical compounds therefore might be able to suppress cancer cells and enzymes. Moreover, these substances markedly decreased the viability of breast cancer cells, even at low concentrations. Additionally, the impacts of breast cancer cells was significantly reduced at a 100 µM dosage of all compounds. The chemical bioactivities of Clusiacyclol A, Cycloolivil, and Cyclopenol against collagenase, hyaluronidase, and elastase were assessed by performing the molecular docking analysis, molecular dynamics (MD) simulation and MM/GBSA calculation. These substances' anti-cancer properties were evaluated about breast cancer cells, including Hs 281.T, SK-BR-3, MCF7, CAMA-1, MDA-MB-468, and NMU. Using in-silico techniques, they examined a few expressed surface receptor proteins, including the androgen receptor, progesterone receptor, estrogen receptor, and CD47.