The Nucleic Acids' Immunoprecipitation Method for DNA Repair Research

Abstract

Typically, the goal of molecular research is to obtain a highly pure target for study. The affinity principle may generally be used to achieve biomolecules that have high purity. Significant benefits come from an antibody’s affinity for its antigen, including the ability to obtain a very pure target antigen and time savings from a single application. Based on the idea of affinity, immunoprecipitation (IP) is a precipitation technique used to purify biomolecules, including proteins, nucleic acids, lipids, and carbohydrates. Nucleic acids are biomolecules responsible for preserving, transferring, and expressing an organism’s genetic information. Therefore, obtaining nucleic acids in high purity is of great importance to scientists. This section covers critical topics such as the basic principles of IP, application types of IP, and the selection of the solid phase and antibodies. In addition, the purification process of repair products resulting from the nucleotide excision repair mechanism of UVC-induced DNA damage is described step by step, serving as an example of nucleic acid purification. © 2025 Elsevier B.V., All rights reserved.