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dc.contributor.authorSurgun-Acar, Yonca
dc.contributor.authorİşkil, Rabia
dc.contributor.authorCeylan, Kevser
dc.contributor.authorCeylan, Yusuf
dc.date.accessioned2019-07-17T06:26:08Z
dc.date.available2019-07-17T06:26:08Z
dc.date.issued2018-11
dc.identifier.urihttp://hdl.handle.net/11772/1658
dc.description.abstractThe aim of the present study is to assess DNA damage in leaves of strawberry (Fragaria × ananassa Duch.) seedlings treated with different heavy metals (zinc, chromium, lead) using random amplified polymorphic DNA (RAPD) assay and analysis of total soluble protein content. For this purpose, strawberry seedlings were treated with 400 and 800 μM Zn, Pb and Cr for 7 days. Thirty four RAPD primers produced 218 bands at molecular weight ranging from 183 bp to 5180 bp. Compared with control, RAPD patterns of heavy metal exposed groups showed differences in band loss, gain of new bands and increase and decrease of band intensity. The highest polymorphism rate (32.11%) was observed in 800 μM Pb applied strawberry seedlings. To evaluate the alterations in RAPD profiles qualitatively, genomic template stability (GTS) was performed and the values were 75.08% and 69.59% for 400 and 800 μM Zn treatments, 74.52% and 68.60% for 400 and 800 μM Cr treatments, 70.11% and 63.78% for 400 and 800 μM Pb treatments, respectively. Total soluble protein content in heavy metal-treated groups also showed a similar correlation to GTS values. RAPD analyses are useful biomarker assays to determine the genotoxicity induced by environmental pollutants such as heavy metals in plant model systems.en_US
dc.language.isoengen_US
dc.publisherFresenius Environmental Bulletinen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBiomarkeren_US
dc.subjectDNA damageen_US
dc.subjectGenomic template stabilityen_US
dc.subjectTo-tal soluble proteinen_US
dc.titleGenotoxicity assessment of heavy metals (zn, cr, pb) on strawberry plants using rapd assayen_US
dc.typearticleen_US
dc.relation.journalFresenius Environmental Bulletinen_US
dc.contributor.departmentBartın Üniversitesi, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümüen_US
dc.identifier.volume27en_US
dc.identifier.issue4en_US
dc.identifier.startpage2483en_US
dc.identifier.endpage2491en_US


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