Effects of different concentrations of bap and naa on micropropagation of crambe orientalis l. var. orientalis l.

Abstract

Crambe L. is a species of the Brassicaceae family. It is an important industrial oil plant with high erusic acid content. The objective of this study was to develop an efficient micropropagation protocol for Crambe orientalis L. var. orientalis L. species using different concentrations of BAP (6 - benzylaminopurine)–NAA (α - naphthalene acetic acid) hormone combinations. Seeds of Crambe orientalis var. orientalis were germinated in vitro. Cotyledon node and hypocotyl parts from fourteen-days seedlings were used as explant sources. The explants were cultured on MS media containing 0.25, 0.50, 1.00 and 2.00 mg/L BAP and 0, 0.25 and 0.50 mg/L NAA. Effects of hormone concentrations on mean callus regeneration percentage (%), mean shoot regeneration percentage (%), mean number of shoots per explant and mean length of shoots were determined. Callus formation and shoot regeneration resulted in all hormone concentrations used for the hypocotyl and cotyledon explants in our study. All explants were rooted on MS media containing 1.00 and 2.00 (mg/L) NAA.