Genotoxicity assessment of heavy metals (zn, cr, pb) on strawberry plants using rapd assay
Özet
The aim of the present study is to assess DNA
damage in leaves of strawberry (Fragaria × ananassa Duch.) seedlings treated with different heavy
metals (zinc, chromium, lead) using random amplified polymorphic DNA (RAPD) assay and analysis
of total soluble protein content. For this purpose,
strawberry seedlings were treated with 400 and 800
μM Zn, Pb and Cr for 7 days. Thirty four RAPD primers produced 218 bands at molecular weight ranging from 183 bp to 5180 bp. Compared with control,
RAPD patterns of heavy metal exposed groups
showed differences in band loss, gain of new bands
and increase and decrease of band intensity. The
highest polymorphism rate (32.11%) was observed
in 800 μM Pb applied strawberry seedlings. To evaluate the alterations in RAPD profiles qualitatively,
genomic template stability (GTS) was performed
and the values were 75.08% and 69.59% for 400 and
800 μM Zn treatments, 74.52% and 68.60% for 400
and 800 μM Cr treatments, 70.11% and 63.78% for
400 and 800 μM Pb treatments, respectively. Total
soluble protein content in heavy metal-treated
groups also showed a similar correlation to GTS
values. RAPD analyses are useful biomarker assays
to determine the genotoxicity induced by
environmental pollutants such as heavy metals in
plant model systems.
