Polymer-Based Transfection Agents Used in CRISPR-CAS9 System

Abstract

Genomeeditingisamethodusedtomakedesiredchangesinthetargetgene.Today, variousmethodsareusedforgenome-editingstudies;amongthem,oneofthemostwidely used methodsistheclustered,regularlyinterspacedshortpalindromicrepeats(CRISPR).CRISPR-associated (Cas) genes and their corresponding CRISPR sequences constitute CRISPR-Cas systems. Due to its simplicity, it is likely that the CRISPR–Cas system could be used effectively in ex vivo genetherapystudiesinhumans.Ifthishappens,theimportanceofCRISPRcarriersystemswill gradually increase. Viral and non-viral systems are used as delivery modalities in genome-editing studies. It has been proven that nanoparticles are the most promising tools for gene therapy due to their adjustablesize, surface, shape, and biologicalbehaviours. Thepolymericcarrier system has becomethemainnon-viralsubstituteforgenedeliveryduetoitsreducedimmunogenicityand pathogenicity. In this review, information about current studies related to polymeric carriers used in non-viral CRISPR delivery systems is presented.