Polymer-Based Transfection Agents Used in CRISPR-CAS9 System
| dc.contributor.author | Imamoglu, Rızvan | |
| dc.contributor.author | Kaplan, Ozlem | |
| dc.contributor.author | Gök, Mehmet Koray | |
| dc.contributor.author | Gokce, Isa | |
| dc.contributor.author | İmamoğlu, Rizvan | |
| dc.date.accessioned | 2025-10-18T08:26:19Z | |
| dc.date.created | 2022 | |
| dc.date.issued | 2022 | |
| dc.department | Bartın Üniversitesi | |
| dc.description.abstract | Genomeeditingisamethodusedtomakedesiredchangesinthetargetgene.Today, variousmethodsareusedforgenome-editingstudies;amongthem,oneofthemostwidely used methodsistheclustered,regularlyinterspacedshortpalindromicrepeats(CRISPR).CRISPR-associated (Cas) genes and their corresponding CRISPR sequences constitute CRISPR-Cas systems. Due to its simplicity, it is likely that the CRISPR–Cas system could be used effectively in ex vivo genetherapystudiesinhumans.Ifthishappens,theimportanceofCRISPRcarriersystemswill gradually increase. Viral and non-viral systems are used as delivery modalities in genome-editing studies. It has been proven that nanoparticles are the most promising tools for gene therapy due to their adjustablesize, surface, shape, and biologicalbehaviours. Thepolymericcarrier system has becomethemainnon-viralsubstituteforgenedeliveryduetoitsreducedimmunogenicityand pathogenicity. In this review, information about current studies related to polymeric carriers used in non-viral CRISPR delivery systems is presented. | |
| dc.identifier.doi | 10.46810/tdfd.795053 | |
| dc.identifier.endpage | 156 | |
| dc.identifier.issn | 2149-6366 | |
| dc.identifier.issue | 1 | |
| dc.identifier.startpage | 151 | |
| dc.identifier.trdizinid | 1069505 | |
| dc.identifier.uri | https://search.trdizin.gov.tr/tr/yayin/detay/1069505 | |
| dc.identifier.uri | https://doi.org/10.46810/tdfd.795053 | |
| dc.identifier.uri | https://hdl.handle.net/11772/18612 | |
| dc.identifier.volume | 11 | |
| dc.indekslendigikaynak | TR-Dizin | |
| dc.language.iso | en | |
| dc.relation.ispartof | Türk Doğa ve Fen Dergisi | |
| dc.relation.publicationcategory | Makale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.snmz | TR-Dizin_20251017 | |
| dc.subject | Hücre ve Doku Mühendisliği | |
| dc.subject | Biyoteknoloji ve Uygulamalı Mikrobiyoloji | |
| dc.subject | CRISPR | |
| dc.subject | polymeric carrier system | |
| dc.subject | non-viraltransfection agent | |
| dc.title | Polymer-Based Transfection Agents Used in CRISPR-CAS9 System | |
| dc.type | Review Article | |
| dspace.entity.type | Publication | |
| relation.isAuthorOfPublication | 48078809-8159-4180-90c4-6675446d47bc | |
| relation.isAuthorOfPublication.latestForDiscovery | 48078809-8159-4180-90c4-6675446d47bc |
